| PES ID | FIFA ID | # | Player | Nationality | Height | Weight | Strong Foot | Team Name(s) |
|---|---|---|---|---|---|---|---|---|
| 37257 | 176094 | 22 | GK Przemysław Tytoń | Poland | 194cm | 83kg | Right | FC Twente Enschede |
| 38400 | 184457 | 9 | CF R. van Wolfswinkel | Netherlands | 185cm | 70kg | Right | FC Twente Enschede |
| 46650 | 199833 | 1 | GK Lars Unnerstall | Germany | 198cm | 103kg | Right | FC Twente Enschede |
| 103205 | 217585 | 5 | LB Bas Kuipers | Netherlands | 180cm | 74kg | Left | FC Twente Enschede |
| 106869 | 208462 | 3 | CB Robin Pröpper | Netherlands | 190cm | 80kg | Right | FC Twente Enschede |
| 110809 | 231864 | 7 | RWF Mitchell van Bergen | Netherlands | 170cm | 62kg | Right | FC Twente Enschede |
| 114558 | 236498 | 10 | CF Sam Lammers | Netherlands | 191cm | 77kg | Left | FC Twente Enschede |
| 115995 | 224163 | 32 | AMF Arno Verschueren | Belgium | 186cm | 73kg | Right | FC Twente Enschede |
| 126744 | 245338 | 20 | CMF Thomas van den Belt | Netherlands | 181cm | 72kg | Right | FC Twente Enschede |
| 127690 | 239371 | 16 | GK Issam El Maach | Morocco | 187cm | 86kg | Right | FC Twente Enschede |
| 128685 | 247931 | 17 | CB Alec Van Hoorenbeeck | Belgium | 187cm | 77kg | Left | FC Twente Enschede |
| 131269 | 262311 | 28 | RB Bart van Rooij | Netherlands | 174cm | 72kg | Right | FC Twente Enschede |
| 132851 | 252383 | 6 | CMF Ramiz Zerrouki | Algeria | 183cm | 74kg | Right | FC Twente Enschede |
| 134924 | 255010 | 37 | LWF Naci Ünüvar | Türkiye | 168cm | 63kg | Right | FC Twente Enschede |
| 135411 | 274927 | 14 | CMF Kristian Hlynsson | Iceland | 174cm | 68kg | Right | FC Twente Enschede |
| 139956 | 259163 | 2 | CB Mees Hilgers | Indonesia | 190cm | 78kg | Right | FC Twente Enschede, Indonesia |
| 139960 | 259563 | 11 | RWF Daan Rots | Netherlands | 179cm | 67kg | Left | FC Twente Enschede |
| 141467 | 259478 | 4 | CMF Mathias Kjølø | Norway | 174cm | 67kg | Right | FC Twente Enschede |
| 141652 | 259673 | 38 | CB Max Bruns | Netherlands | 183cm | 67kg | Right | FC Twente Enschede |
| 146621 | 256887 | 8 | CMF Taylor Booth | United States | 173cm | 66kg | Right | FC Twente Enschede |
| 154146 | 269232 | 21 | GK Sam Karssies | Netherlands | 186cm | 78kg | Right | FC Twente Enschede |
| 160470 | 274834 | 39 | LB Mats Rots | Netherlands | 185cm | 78kg | Left | FC Twente Enschede |
| 160471 | - | 41 | DMF Gijs Besselink | Netherlands | 180cm | 69kg | Right | FC Twente Enschede |
| 168116 | 70539 | 25 | CF L. Vennegoor of H. | Netherlands | 188cm | 78kg | Right | FC Twente Enschede |
| 177358 | - | 23 | CB Stav Lemkin | Israel | 190cm | 80kg | Right | FC Twente Enschede |
| 177790 | - | 12 | RB Guilherme Peixoto | Portugal | 180cm | 75kg | Right | FC Twente Enschede |