| PES ID | FIFA ID | # | Player | Nationality | Height | Weight | Strong Foot | Team Name(s) |
|---|---|---|---|---|---|---|---|---|
| 60463 | - | 31 | RB Steven Moreira | Cabo Verde | 179cm | 75kg | Right | Columbus BY |
| 111231 | 234300 | 26 | LWF Lassi Lappalainen | Finland | 184cm | 80kg | Right | Columbus BY |
| 112103 | - | 6 | CMF Darlington Nagbe | United States | 175cm | 75kg | Right | Columbus BY |
| 119476 | - | 30 | CB Rudy Camacho | France | 185cm | 79kg | Right | Columbus BY |
| 121372 | 245855 | 7 | AMF Dylan Chambost | France | 177cm | 70kg | Left | Columbus BY |
| 128044 | 242263 | 18 | LB Malte Amundsen | Denmark | 179cm | 81kg | Left | Columbus BY |
| 130317 | 238277 | 8 | AMF Dániel Gazdag | Hungary | 178cm | 76kg | Right | Columbus BY, Hungary |
| 142946 | 206549 | 24 | GK Evan Bush | United States | 188cm | 82kg | Right | Columbus BY |
| 143016 | - | 14 | DMF Amar Sejdić | United States | 178cm | 72kg | Right | Columbus BY |
| 143076 | - | 10 | LWF Diego Rossi | Uruguay | 170cm | 65kg | Right | Columbus BY |
| 143090 | - | 20 | CMF Derrick Jones | United States | 190cm | 80kg | Right | Columbus BY |
| 145759 | - | 13 | AMF AZ Jackson | United States | 175cm | 70kg | Right | Columbus BY |
| 152815 | 266389 | 28 | GK Patrick Schulte | United States | 195cm | 90kg | Left | Columbus BY |
| 153033 | 266390 | 25 | CMF Sean Zawadzki | United States | 183cm | 73kg | Right | Columbus BY |
| 155768 | 269923 | 19 | CF Jacen Russell-Rowe | Canada | 183cm | 84kg | Right | Columbus BY |
| 156793 | - | 23 | RB Mohamed Farsi | Algeria | 180cm | 69kg | Right | Columbus BY |
| 160498 | - | 27 | RWF Max Arfsten | United States | 185cm | 78kg | Left | Columbus BY |
| 162153 | - | 11 | CF Ibrahim Aliyu | Nigeria | 184cm | 75kg | Right | Columbus BY |
| 162995 | - | 16 | CMF Taha Habroune | United States | 182cm | 76kg | Right | Columbus BY |
| 164480 | - | 21 | CB Yevgen Cheberko | Ukraine | 184cm | 73kg | Left | Columbus BY |
| 168372 | - | 1 | GK Nicholas Hagen | Guatemala | 193cm | 80kg | Right | Columbus BY, Guatemala |
| 168772 | - | 29 | CMF Cole Mrowka | United States | 183cm | 75kg | Right | Columbus BY |
| 168933 | - | 41 | GK Stanislav Lapkes | Belarus | 194cm | 82kg | Right | Columbus BY |